anti-CCNE1 antibody product blog
Tags: Antibody; CCNE1; anti-CCNE1 antibody; Polyclonal Antibody; Cyclin E1;
The CCNE1 ccne1 (Catalog #MBS8226961) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclin E1 (pT395) Phospho Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Cyclin E1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB (1/500 - 1/1000), IHC (1/100 - 1/200). Researchers should empirically determine the suitability of the CCNE1 ccne1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CCNE1 ccne1 product has the following accession number(s) (GI #17318559) (NCBI Accession #NP_001229.1) (Uniprot Accession #P24864). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Cyclin E1 (pT395) Phospho Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Cyclin E1 (pT395) expression in HepG2 (A), JAR (B) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of Cyclin E1 (pT395) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Rabbit polyclonal antibody to Cyclin E1 (pT395).
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cyclin E1. The exact sequence is proprietary. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CCNE1 are readily searchable from our website. Different antibodies against the same target such as CCNE1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. CCNE1 also interacts with the following gene(s): CDC25A, CDK1, CDK2, CDKN1A, CDKN1B, E2F3, FBXW7, RB1. Embryonic Tissue, Heart, Intestine, Kidney, Liver, Lung, Ovary, Prostate, Skin, Testis tissues are correlated with this protein. Adenocarcinoma, Breast Neoplasms, Carcinoma, Hepatocellular, Cell Transformation, Neoplastic, Disease Progression, Liver Diseases, Liver Neoplasms, Lung Neoplasms, Neoplasms, Experimental, Ovarian Neoplasms are some of the diseases may be linked to Anti-Cyclin E1 (pT395) Phospho Antibody.