|The CD274 cd274 (Catalog #MBS176279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD274 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CD274 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CD274 cd274 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD274 cd274 product has the following accession number(s) (GI #83287884) (NCBI Accession #Q9NZQ7.1) (Uniprot Accession #Q9NZQ7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-CD274 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-CD274 antibody, MBS176279, Western blotting
All lanes: Anti CD274 (MBS176279) at 0.5ug/ml
Lane 1: Rat Heart Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 70KD)
Description: Rabbit IgG polyclonal antibody for Programmed cell death 1 ligand 1(CD274) detection. Tested with WB in Human, Mouse, Rat.
Background: CD274(CD274 molecule), also known as PDCD1L1, PDL1, B7H1, CD274 molecule, cluster of differentiation(CD274) or B7 homolog 1(B7-H1), Programmed cell death 1 ligand 1, is a protein that in humans is encoded by the CD274 gene. Levels of B7H1 correlated with PTEN loss in glioblastoma specimens, and tumor-specific T cells lysed human glioma targets expressing wildtype PTEN more effectively than those expressing mutant PTEN. Sequence analysis predicted that it has 290-amino acid. immunoresistance in glioma is related to loss of the tumor suppressor PTEN and is mediated in part by B7H1.Programmed death ligand 1(PD-L1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Using microarray analysis and flow cytometry, Barber et al.(2006) found that Pd1 was highly upregulated by functionally exhausted CD8 T cells from mice infected with a lymphocytic choriomeningitis virus(LCMV) strain causing chronic infection, but not by functional memory CD8 T cells from mice infected with an LCMV strain causing acute infection. .
Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CD274 (100-114aa QITDVKLQDAGVYRC), different from the related rat and mouse sequences by one amino acid. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.