anti-CD59 antibody product blog
Tags: Antibody; Polyclonal Antibody; CD59; anti-CD59 antibody;
The CD59 cd59 (Catalog #MBS126594) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD59 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CD59 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the CD59 cd59 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD59 cd59 product has the following accession number(s) (GI #116021) (NCBI Accession #P13987.1) (Uniprot Accession #P13987). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD59 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CD59 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse brain using CD59 antibody (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse lung using CD59 antibody (40x lens).)
This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008].
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human CD59
Calculated Molecular Weight: 19kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD59 are readily searchable from our website. Different antibodies against the same target such as CD59 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.