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anti-CDH11 antibody product blog

Posted on 2015-08-11 05:15:53 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; CDH11; anti-CDH11 antibody;
The CDH11 cdh11 (Catalog #MBS422381) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CDH11 (aa 497-509) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. MyBioSource\'s CDH11 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB-Trf).
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: In transfected HEK293 transiently expressing full-length Human CDH11 (myc and DYKDDDDK tagged), a band of approx.130kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the CDH11 cdh11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CDH11 cdh11 product has the following accession number(s) (GI #16306532) (NCBI Accession #NP_001788.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-CDH11 (aa 497-509) Antibody with the following immunoassay(s):
Western Blot (WB) (HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CDH11 with C-terminal MYC tag probed with (1ug/ml) in Lane An and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Western Blot (WB) CDH11.

Immunogen: Peptide with sequence CESDQTKPLSNQP, from the internal region of the protein sequence according to NP_001788.2.
Epitope: Internal region. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CDH11 are readily searchable from our website. Different antibodies against the same target such as CDH11 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. CDH11 also interacts with the following gene(s): CDH13, CDH15, CDH2, CDH3, CDH4, CDH5. Breast Neoplasms, Carcinoma, Cell Transformation, Neoplastic, Disease Models, Animal, Fibrosis, Inflammation, Lung Diseases, Lung Neoplasms, Neoplasm Metastasis, Skin Neoplasms are some of the diseases may be linked to Goat anti-CDH11 (aa 497-509) Antibody. Bone, Brain, Embryonic Tissue, Eye, Lung, Muscle, Placenta, Prostate, Skin, Vascular tissues are correlated with this protein.
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