anti-CDK16 antibody product blog
Tags: Antibody; Polyclonal Antibody; CDK16; anti-CDK16 antibody; PCTAIRE 1;
The CDK16 cdk16 (Catalog #MBS618539) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PCTAIRE 1 reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s PCTAIRE 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS).Suitable for use in Western Blot, Flow Cytometry and Immunoprecipitation.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Flow Cytometry: 1:50. Researchers should empirically determine the suitability of the CDK16 cdk16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CDK16 cdk16 product has the following accession number(s) (GI #15990456) (NCBI Accession #AAH15607.1) (Uniprot Accession #Q00536). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PCTAIRE 1 with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from CAD and Neuro2A cells, using MBS618539.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of untreated Neuro 2A cells, using MBS618539 (blue) compared to a nonspecific negative control antibody (red).)
Three distinct PCTAIRE isoforms (PCTAIRE 1, PCTAIRE 2 and PCTAIRE 3) have been identified in humans and belong to the Cdk family of serine/threonine protein kinases. These proteins have a core kinase domain flanked by unique amino-and carboxy- terminal domains. Cdk proteins are known to regulate the cell cycle. All three PCTAIRE isoforms are abundantly expressed and catalytically active in post-mitotic brain, suggesting that they may function in processes other than cell division (1). PCTAIRE 1 is a cytoplasmic phosphoprotein whose kinase activity peaks in G2 and S phase (2). While one study indicates that noncovalent interactions with a regulatory component (such as a cyclin) are necessary for catalytic activity of PCTAIRE 1, others show that the monomeric protein is fully active (3). The Cdk5/p25 complex phosphorylates PCTAIRE 1 at Ser95, enhancing its kinase activity (4).
Immunogen: Synthetic peptide derived from the sequence of human PCTAIRE 1 (KLH-coupled). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CDK16 are readily searchable from our website. Different antibodies against the same target such as CDK16 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.