|The CEM15 apobec3g (Catalog #MBS243586) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human CEM15 / APOBEC3G reacts with Human
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. MyBioSource\'s CEM15 / APOBEC3G can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA).
ELISA (1:16000), IHC-P (2.5 ug/ml), WB (0.5 - 1.5 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 45kD band observed in lysates of cell line Daudi (calculated MW of 46.4kD according to NP_068594.1). Recommended concentration: 0.5-1.5 ug/ml. Researchers should empirically determine the suitability of the CEM15 apobec3g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CEM15 apobec3g product has the following accession number(s) (GI #13399304) (NCBI Accession #NP_068594.1) (Uniprot Accession #Q9HC16). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat Polyclonal to Human CEM15 / APOBEC3G with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-APOBEC3G antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)
Immunohistochemistry (IHC) (Anti-APOBEC3G antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)
Western Blot (WB) (Antibody (0.5 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates.
Target Species: Human
Immunogen Description: Synthetic peptide C-DEHSQDLSGRLR from the C-terminus of human APOBEC3G (NP_068594.1). Percent identity by BLAST analysis: Human, Baboon (100%); Monkey (92%); Chimpanzee, Gorilla, Gibbon, Marmoset (83%). Immunogen Type: Synthetic peptide
Immunogen: CEM15 / APOBEC3G antibody was raised against synthetic peptide C-DEHSQDLSGRLR from the C-terminus of human APOBEC3G (NP_068594.1). Percent identity by BLAST analysis: Human, Baboon (100%); Monkey (92%); Chimpanzee, Gorilla, Gibbon, Marmoset (83%).
Antigen Modification: C-Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CEM15 are readily searchable from our website. Different antibodies against the same target such as CEM15 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.