anti-Cenpb antibody product blog
Tags: Antibody; CENPB; Polyclonal Antibody; anti-CENPB antibody;
The Cenpb cenpb (Catalog #MBS644002) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CENPB (Major Centromere Autoantigen B, Centromere Protein B, CENP-B) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CENPB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP).Suitable for use in Western Blot and Immunoprecipitation. Researchers should empirically determine the suitability of the Cenpb cenpb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Cenpb cenpb product has the following accession number(s) (GI #13879308) (NCBI Accession #AAH06628.1) (Uniprot Accession #P27790). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
MGPKRRQLTF REKSRIIQEV EENPDLRKGE IARRFNIPPS TLSTILKNKR AILASERKYG VASTCRKTNK LSPYDKLEGL LIAWFQQIRA AGLPVKGIIL KEKALRIAEE LGMDDFTASN GWLDRFRRRH GVVSCSGVAR ARARNAAPRT PAAPASPAAV PSEGSGGSTT GWRAREEQPP SVAEGYASQD VFSATETSLW YDFLPDQAAG LCGGDGRPRQ ATQRLSVLLC ANADGSEKLP PLVAGKSAKP RAGQAGLPCD YTANSKGGVT TQALAKYLKA LDTRMAAESR RVLLLAGRLA AQSLDTSGLR HVQLAFFPPG TVHPLERGVV QQVKGHYRQA MLLKAMAALE GQDPSGLQLG LTEALHFVAA AWQAVEPSDI AACFREAGFG GGPNATITTS LKSEGEEEEE EEEEEEEEEG EGEEEEEEGE EEEEEGGEGE ELGEEEEVEE EGDVDSDEEE EEDEESSSEG LEAEDWAQGV VEAGGSFGAY GAQEEAQCPT LHFLEGGEDS DSDSEEEDDE EEDDEDEDDD DDEEDGDEVP VPSFGEAMAY FAMVKRYLTS FPIDDRVQSH ILHLEHDLVH VTRKNHARQA GVRGLGHQS.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CENPB (Major Centromere Autoantigen B, Centromere Protein B, CENP-B) with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of CENPB expression in transfected 293T cell line by CENPB polyclonal antibody. Lane 1: CENPB transfected lysate (65.2kD). Lane 2: Non-transfected lysate.)
Immunoprecipitation (IP) (Immunoprecipitation of CENPB transfected lysate using CENPB rabbit polyclonal antibody and Protein A Magnetic Bead and immunoblotted with CENPB purified mouse polyclonal antibody.)
This gene product is a highly conserved protein associated with the centromere. It is a DNA-binding protein containing a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
Immunogen: Full length human CENPB, aa1-599 (AAH53847.1). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Cenpb are readily searchable from our website. Different antibodies against the same target such as Cenpb may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.