anti-CFLAR antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-CFLAR antibody; CFLAR;
The CFLAR cflar (Catalog #MBS2525044) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CFLAR Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CFLAR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the CFLAR cflar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The CFLAR cflar product has the following accession number(s) (GI #12862674) (NCBI Accession #BAB32551.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CFLAR Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various tissues, using CFLAR antibody.)
Immunohistochemistry (IHC) (Immunohistochemical of paraffin-embedded rat testis using CFLAR antibody at dilution of 1:200 (200x lens).)
Cellular FLIP (FLICE inhibitory protein) is a regulator of apoptosis that has various names, such as c-FLIP (1), Casper (2), CLARP (3), FLAME (4), I-FLICE (5), MRIT (6), CASH (7), and Usurpin (8). FLIP is expressed as two alternative splice isoforms, FLIP short (FLIPS) and FLIP long (FLIPL). FLIPS contains two death effector domains (DEDs) like those found on the death receptor adaptor protein FADD and the pro-domain of caspase-8. FLIPL shares significant homology with caspase-8 (FLICE), and contains an additional death effector domain, but FLIPL lacks the catalytic active site of the caspases and does not have protease activity. Both FLIP isoforms have been reported to interact with FADD and pro-caspase-8. The role of FLIP in apoptosis is controversial as some research studies have reported it to be anti-apoptotic, while others claim that it is pro-apoptotic. Overexpression of FLIPL can lead to caspase-8 heterodimers that produce an active protease, resulting in apoptosis. However, at physiological levels, it is thought that the binding of FLIP to the DED of FADD results in inhibition of caspase-8 processing. Reduction of FLIP by siRNA or gene targeting sensitizes cells to death receptor-mediated apoptosis. FLIP has also been implicated in the resistance of cancer cells to apoptosis and is upregulated in some cancer types including Hodgkin\'s lymphoma and ovarian and colon carcinomas (9).
Immunogen: Recombinant protein of human CFLAR
Observed Molecular Weight: 52kDa. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7109 / sc-7108 / sc-7111 / sc-8346 / sc-8347. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CFLAR are readily searchable from our website. Different antibodies against the same target such as CFLAR may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.