anti-CFP antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-CFP antibody; CFP; Properdin;
The CFP cfp (Catalog #MBS573234) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti Human properdin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s properdin can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP).Applications: Immunoprecipitation. In immunoelectrophoresis use 2 ul human serum against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in a 3 mm diameter center well and 10 ul of the antiserum in 3 mm diameter peripheral wells. In single radial immunodiffusion use 1% antiserum in the gel. Researchers should empirically determine the suitability of the CFP cfp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CFP cfp product has the following accession number(s) (GI #4505737) (NCBI Accession #NP_002612.1) (Uniprot Accession #P27918). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The defined antibody specificity is restricted to properdin as tested at the level of sensitivity of immunoprecipitation techniques. A single precipitin line is obtained in immuno- electrophoresis, bidimensional electrophoresis and double radial immunodiffusion (Ouchterlony) against normal plasma, which shows a reaction of identity with the precipitated purified properdin. No reaction is obtained with any other plasma protein. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.
Product type: Primary Antibodies
Source Note: Properdin is a factor of the alternative complement activation pathway. It has a molecular weight of 222,000 and consists of four peptide chains. Properdin is stabilizing the C3 convertase C3bBb. The mean concentration in plasma is 20 mg/ml. The antigen has been isolated form pooled human plasma. Freund�s complete adjuvant is used in the first step of the immunization procedure.
Product: Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Reactivity Note: The antiSerum does not cross react with any other component of Human plasma. Inter- species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CFP are readily searchable from our website. Different antibodies against the same target such as CFP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.