anti-CHEK2 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-CHEK2 antibody; CHEK2;
The CHEK2 chek2 (Catalog #MBS126984) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHEK2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CHEK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorecence (IF), Immunoprecipitation (IP).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
ICC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:100. Researchers should empirically determine the suitability of the CHEK2 chek2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CHEK2 chek2 product has the following accession number(s) (GI #6685284) (NCBI Accession #O96017.1) (Uniprot Accession #O96017). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CHEK2 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CHEK2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Immunofluorescence (IF) (Immunofluorescence analysis of MCF-7 cells using CHEK2 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF) (Immunofluorescence analysis of A549 cells using CHEK2 antibody.)
Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8).
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human CHEK2
Calculated Molecular Weight: 61kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CHEK2 are readily searchable from our website. Different antibodies against the same target such as CHEK2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.