anti-CHEK2 antibody product blog
Tags: Antibody; Polyclonal Antibody; Chk2; anti-CHEK2 antibody; CHEK2;
The CHEK2 chek2 (Catalog #MBS151546) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Chk2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Chk2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC).Chk2 antibody can be used for detection of Chk2 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 1 mug/mL. Researchers should empirically determine the suitability of the CHEK2 chek2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CHEK2 chek2 product has the following accession number(s) (GI #6005850) (NCBI Accession #NP_009125) (Uniprot Accession #O96017). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Chk2 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Chk2 expression in (A) K562, (B) Jurkat, and (C) HL-60 whole cell lysates with Chk2 antibody at 1 μg /ml.)
Immunocytochemistry (IHC) (Immunocytochemistry of Chk2 in Jurkat cells with Chk2 antibody at 1 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of Chk2 in Jurkat cells with Chk2 antibody at 5 μg/mL
Red: Chk2 Antibody (2391)
Blue: DAPI staining)
Chk2 Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.
Conjugate: Unconjugated
Immunogen: Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2.
Buffer: Chk2 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CHEK2 are readily searchable from our website. Different antibodies against the same target such as CHEK2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.