|The CHUK chuk (Catalog #MBS625679) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IkB alpha, phosphorylated (Tyr42) (IkBa) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s IkB alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC).
Dilution: Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the CHUK chuk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CHUK chuk product has the following accession number(s) (GI #62241001) (NCBI Accession #NP_001269.3) (Uniprot Accession #O15111). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
NFkB exists in the cytoplasm as a complex with a 38kD inhibitor protein, IkB (MAD-3). Activation of NFkB by different stimuli, including TNFa and PMA, requires the dissociation of the inhibitor protein. Dissociation is regulated by the phosphorylation of IkB on serine residues 32 and 36 which targets IkB for ubiquinitation and proteasome mediated degradation. The NFkB complex then translocates to the nucleus and activates transcription of responsive genes.
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Immunogen: Synthesized phosphopeptide derived from human IkBa around the phosphorylation site of tyrosine 42 (E-E-YP-E-Q). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CHUK are readily searchable from our website. Different antibodies against the same target such as CHUK may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Embryonic Tissue, Heart, Kidney, Liver, Lung, Muscle, Skin tissues are correlated with this protein. CHUK also interacts with the following gene(s): AKT1, IKBKB, IKBKG, NFKB1, NFKB2, NFKBIA, NFKBIB, RELA. The following patways have been known to be associated with this gene. Abnormalities, Multiple, Adenocarcinoma, Cell Transformation, Neoplastic, Heart Diseases, Inflammation, Liver Diseases, Liver Neoplasms, Necrosis, Neoplasms, Skin Abnormalities are some of the diseases may be linked to IkB alpha, phosphorylated (Tyr42) (IkBa).