anti-CLDN1 antibody product blog
Tags: Antibody; Polyclonal Antibody; CLDN1; anti-CLDN1 antibody;
The CLDN1 cldn1 (Catalog #MBS150617) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLDN1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CLDN1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).CLDN1 antibody can be used for detection of CLDN1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the CLDN1 cldn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CLDN1 cldn1 product has the following accession number(s) (GI #48146393) (NCBI Accession #CAG33419) (Uniprot Accession #O95832). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CLDN1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of CLDN1 in HepG2 cell lysate with CLDN1 antibody at (A) 1 and (B) 2 μg/mL.)
Immunocytochemistry (IHC) (Immunocytochemistry of CLDN1 in HepG2 cells with CLDN1 antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of CLDN1 in HepG2 cells with CLDN1 antibody at 20 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of CLDN1 in HepG2 cells with CLDN1 antibody at 20 μg/mL.
Green: CLDN1 Antibody (5187)
Blue: DAPI staining)
CLDN1 Antibody: Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions are specialized regions of cell to cell contact consisting of networking strands that act as a molecular gasket for preventing the leakage of ions, water, etc., between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. CLDN1 and CLDN2 were found to be overexpressed in colonal cancer tissues and may be useful as tumor markers and targets for the treatment of colorectal cancer. Characterization of Claudins expression in human tumors can be an additional tool. Recent studies show that CLDN1 has gastric tumor suppressive activity and is a direct transcriptional target of RUNX3. Along with SCARB1, LDL-R, and the tetraspanin superfamily member CD81, CLDN1 has been reported to be an entry factor for the Hepatitis C virus.
Conjugate: Unconjugated
Immunogen: CLDN1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human CLDN1.
Buffer: CLDN1 Antibody is supplied in PBS containing 0.02% sodium azide.