|The CLDN3 cldn3 (Catalog #MBS8238113) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Claudin 3 Antibody reacts with Human, Bovine and may cross-react with other species as described in the data sheet. MyBioSource\'s Claudin 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP).
WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF (1/100 - 1/500), ICC (1/100 - 1/500), IP (1/10 - 1/100). Researchers should empirically determine the suitability of the CLDN3 cldn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CLDN3 cldn3 product has the following accession number(s) (GI #4502875) (NCBI Accession #NP_001297.1) (Uniprot Accession #O15551). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Claudin 3 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Claudin 3 expression in SW480 (A), LOVO (B) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of Claudin 3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. w)
Immunofluorescence (IF) (Immunofluorescent analysis of Claudin 3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Rabbit polyclonal antibody to Claudin 3.
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Claudin 3. The exact sequence is proprietary. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CLDN3 are readily searchable from our website. Different antibodies against the same target such as CLDN3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.