anti-CNTF antibody product blog
Tags: Antibody; Polyclonal Antibody; CNTF; anti-CNTF antibody;
The CNTF cntf (Catalog #MBS176996) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CNTF Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CNTF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). Researchers should empirically determine the suitability of the CNTF cntf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The CNTF cntf product has the following accession number(s) (GI #116587) (NCBI Accession #P20294.1) (Uniprot Accession #P20294). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-CNTF Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-CNTF Picoband antibody, MBS176996-1.JPG
IHC(P): Mouse Brain Tissue )
Immunohistochemistry (IHC) (Anti-CNTF Picoband antibody, MBS176996-2.JPG
IHC(P): Rat Brain Tissue )
Testing Data (Anti-CNTF Picoband antibody, MBS176996-3.jpg
All lanes: Anti-CNTF(MBS176996) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: NRK Whole Cell Lysate at 40ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Lane 4: Rat Brain Tissue Lysate at 40ug
Lane 5: Rat Liver Tissue Lysate at 40ug
Lane 6: Rat Spleen Tissue Lysate at 40ug
Lane 7: Rat Testis Tissue Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD)
Description: Rabbit IgG polyclonal antibody for Ciliary neurotrophic factor(CNTF) detection. Tested with WB, IHC-P, ELISA in Mouse, Rat.
Background: Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene. The protein encoded by this gene is a polypeptide hormone andnerve growth factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neural populations includingastrocytes. It is mapped to 11q12.1. In addition to neurotrophic effects on parasympathetic neurons, CNTF was shown to have activities on sympathetic and sensory neurons. CNTF appears not to be involved in motor neuron survival during development. human CNTF has a trophic influence on degenerating striatal neurons as well as on critical nonstriatal regions. CNTF plays a role in a cardiac signal transduction pathway that regulates obesity-related left ventricular hypertrophy.
Immunogen: E Coli-derived rat CNTF recombinant protein (Position: A2-M200). Rat CNTF shares 84% and 95% amino acid (aa) sequences identity with human and mouse CNTF, respectively. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CNTF are readily searchable from our website. Different antibodies against the same target such as CNTF may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.