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anti-COL3A1 antibody product blog

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Posted on 2015-12-27 16:24:58 by mybiosource_staff
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Tags: Antibody; COL3A1; Polyclonal Antibody; anti-COL3A1 antibody; COLLAGEN III;
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The COL3A1 col3a1 (Catalog #MBS223554) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI HUMAN COLLAGEN III reacts with Mammals and may cross-react with other species as described in the data sheet. MyBioSource\'s COLLAGEN III can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)*.
ELISA: Minimum Dilution: 1/5000; Maximum Dilution: 1/10000;
Immunohistology - Paraffin: Maximum Dilution: 2.5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 1/5000; Maximum Dilution: 1/10000; Application Note: This product is suitable for use in Western Blotting under non-reducing conditions. Researchers should empirically determine the suitability of the COL3A1 col3a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The COL3A1 col3a1 product has the following accession number(s) (GI #4502951) (NCBI Accession #NP_000081.1) (Uniprot Accession #P02461). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RABBIT ANTI HUMAN COLLAGEN III with the following immunoassay(s):
Testing Data #1 (Staining of formalin fixed, paraffin embedded human testis with Rabbit anti Human collagen III (MBS223554))
Testing Data #1 COL3A1.

Testing Data #2 (Staining of formalin fixed, paraffin embedded human kidney with Rabbit anti Human collagen III (MBS223554))
Testing Data #2 COL3A1.

MBS223554 recognises Human Collagen III (COL3A1), a secreted protein of 1466 amino acids belonging to the fibrillar collagen family. Collagens are highly conserved across species and are vital in the formation of connective tissues. Collagen III is found in most soft connective tissues along with Collagen I. Defects in collagen III are associated with Ehlers-Danlos syndrome type 3 and type 4 and with aortic aneurysm abdominal (AAA).

Perservative Stabilisers: 0.01% Sodium Azide (NaN3). Immunogen: Synthetic peptide corresponding to Human collagen III
Histology Positive Control Tissue: Human kidney
Buffer Solution: pH8.0, 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA
Antiserum Preparation: Antisera to human Collagen III were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing COL3A1 are readily searchable from our website. Different antibodies against the same target such as COL3A1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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