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anti-COL4A1 antibody product blog

Posted on 2018-03-03 10:33:44 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; anti-COL4A1 antibody; COL4A1; Collagen alpha-1 (IV) chain;
The COL4A1 col4a1 (Catalog #MBS7005956) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Collagen alpha-1(IV) chain polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Collagen alpha-1 (IV) chain can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC).
Recommended dilution: IHC: 1:20-1:200. Researchers should empirically determine the suitability of the COL4A1 col4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The COL4A1 col4a1 product has the following accession number(s) (GI #734520332) (NCBI Accession #NP_001290039.1) (Uniprot Accession #P02462). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti-human Collagen alpha-1(IV) chain polyclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung using MBS7005956 at dilution of 1:100)
Immunohistochemistry (IHC) COL4A1.

Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7005956 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF) COL4A1.

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a \'chicken-wire\' meshwork together with laminins, proteoglycans and entactin/nidogen. Ref.12 Ref.17 Ref.18 Ref.19

This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.

Immunogen: Recombinant human Collagen alpha-1(IV) chain protein. Conjugation: Non-conjugated
Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9301 / sc-9302. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing COL4A1 are readily searchable from our website. Different antibodies against the same target such as COL4A1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. COL4A1 also interacts with the following gene(s): COL12A1, COL4A2, COL4A6, COL7A1, COL8A1, ITGB1, NID1, PTK2.
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