anti-COL4a4 antibody product blog
Tags: Antibody; Polyclonal Antibody; COL4A4; anti-COL4A4 antibody; Collagen Type IV Alpha 4;
The COL4a4 col4a4 (Catalog #MBS2032561) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen Type IV Alpha 4 (COL4a4) Polyclonal Antibody reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. MyBioSource\'s Collagen Type IV Alpha 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the COL4a4 col4a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The COL4a4 col4a4 product has the following accession number(s) (GI #116256356) (NCBI Accession #NP_000083.3) (Uniprot Accession #P53420). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Collagen Type IV Alpha 4 (COL4a4) Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Figure. Western Blot; Sample: Recombinant COL4a4, Human.)
Western Blot (WB) (Western Blot; Sample:
Lane1: 293T cell lysate;
Lane2: Hela cell lysate
Primary Ab: 2ug/ml Rabbit Anti-Human COL4a4 Antibody
Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: MBS2086047))
Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Kidney Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human COL4a4 Antibody
Second Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: MBS2086047))
Source: Polyclonal antibody preparation. Traits: Liquid
Immunogen: Recombinant COL4a4 (Val1469~Ser1690) expressed in E.coli (MBS2033309). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing COL4a4 are readily searchable from our website. Different antibodies against the same target such as COL4a4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Connective Tissue, Eye, Kidney, Liver, Muscle, Placenta, Stomach, Testis tissues are correlated with this protein. COL4a4 also interacts with the following gene(s): FN1, MMP9. The following patways have been known to be associated with this gene. Alport syndrome, recessive type, Brain Diseases, Hemorrhage, Inflammation, Kidney Diseases, Necrosis, Neoplasms, Nervous System Diseases, Proteinuria, Renal Insufficiency are some of the diseases may be linked to Collagen Type IV Alpha 4 (COL4a4) Polyclonal Antibody.