anti-COX5B antibody product blog
Tags: Antibody; Polyclonal Antibody; COX5B; anti-COX5B antibody;
The COX5B cox5b (Catalog #MBS641200) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COX5B, ID (COX5B, Cytochrome c oxidase subunit 5B, mitochondrial, Cytochrome c oxidase polypeptide Vb) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s COX5B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500. Researchers should empirically determine the suitability of the COX5B cox5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The COX5B cox5b product has the following accession number(s) (GI #17017988) (NCBI Accession #NP_001853.2) (Uniprot Accession #P10606). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the COX5B, ID (COX5B, Cytochrome c oxidase subunit 5B, mitochondrial, Cytochrome c oxidase polypeptide Vb) with the following immunoassay(s):
Western Blot (WB) (COX5B Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the COX5B antibody detected the COX5B protein (arrow).)
Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.
Immunogen: COX5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-49 amino acids from the Central region of human COX5B. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing COX5B are readily searchable from our website. Different antibodies against the same target such as COX5B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.