anti-CTSE antibody product blog
Tags: Antibody; Polyclonal Antibody; Ctse; anti-CTSE antibody;
The CTSE ctse (Catalog #MBS624238) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CTSE, ID (Cathepsin E, Cathepsin E form I, Cathepsin E form II) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CTSE can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the CTSE ctse for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CTSE ctse product has the following accession number(s) (GI #4503145) (NCBI Accession #NP_001901.1) (Uniprot Accession #P14091). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CTSE, ID (Cathepsin E, Cathepsin E form I, Cathepsin E form II) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of CTSE antibody (Center) in K562 cell line lysates (35ug/lane).CTSE(arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of CTSE antibody (Center) in mouse stomach tissue lysates (35ug/lane).CTSE(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (CTSE Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CTSE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (CTSE Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CTSE is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin An and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen.
Immunogen: Synthetic peptide selected from the Center region of human CTSE (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CTSE are readily searchable from our website. Different antibodies against the same target such as CTSE may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.