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anti-CUL2 antibody product blog

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Posted on 2018-04-03 15:09:09 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; CUL2; anti-CUL2 antibody; Cullin 2;
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The CUL2 cul2 (Catalog #MBS9412061) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cullin 2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Cullin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500. Researchers should empirically determine the suitability of the CUL2 cul2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CUL2 cul2 product has the following accession number(s) (GI #311771637) (NCBI Accession #NP_001185706.1) (Uniprot Accession #Q13617). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Cullin 2 Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cullin 2 antibody.)
Immunohistochemistry (IHC) CUL2.

Immunofluorescence (IF) (Immunofluorescence analysis of NIH/3T3 cells, using Cullin 2 antibody.)
Immunofluorescence (IF) CUL2.

Western Blot (WB) (Western blot analysis of extracts from LOVO cells, using Cullin 2 antibody.)
Western Blot (WB) CUL2.

Western Blot (WB) (Western blot analysis of extracts from HepG2 cells (Lane 2), using Cullin 2 antiobdy. The lane on the left is treated with systhesized peptide.)
Western Blot (WB) CUL2.

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 By similarity. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).Seiji Shiraishi, J. Biol. Chem., Mar 2007; 282: 9017 - 9028.Isabelle Groulx, J. Biol. Chem., Mar 2000; 275: 8991.Dimitris Liakopoulos, PNAS, May 1999; 96: 5510.Kyungwon Huh, AACR Meeting Abstracts, Apr 2006; 2006: 1326.

Immunogen Type: Peptide
Immunogen Description: Synthesized peptide derived from human Cullin 2. Target Name: Cullin 2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CUL2 are readily searchable from our website. Different antibodies against the same target such as CUL2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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