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anti-CYLD antibody product blog

Posted on 2015-12-01 01:28:38 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; Cyld; anti-CYLD antibody;
The CYLD cyld (Catalog #MBS128593) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYLD Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CYLD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).
WB: 1:200-1:2000
IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CYLD cyld for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CYLD cyld product has the following accession number(s) (GI #51316104) (NCBI Accession #Q9NQC7.1) (Uniprot Accession #Q9NQC7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CYLD Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CYLD antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.)
Western Blot (WB) CYLD.

This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human CYLD
Calculated Molecular Weight: 107kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CYLD are readily searchable from our website. Different antibodies against the same target such as CYLD may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Bone Marrow, Ear, Kidney, Liver, Lung, Skin, Thymus, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. Adenocarcinoma, Carcinoma, Hepatocellular, Carcinoma, Squamous Cell, Cell Transformation, Neoplastic, Familial cylindromatosis, Gastrointestinal Diseases, Inflammation, Liver Diseases, Necrosis, Trichoepithelioma multiple familial are some of the diseases may be linked to CYLD Polyclonal Antibody. CYLD also interacts with the following gene(s): DDX58, IKBKG, RIPK1, SQSTM1, TNFRSF1A, TRAF2, TRAF6, TRAIP.
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