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anti-CYP3A5 antibody product blog

Posted on 2015-10-25 15:35:10 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; CYP3A5; anti-CYP3A5 antibody;
The CYP3A5 cyp3a5 (Catalog #MBS9204369) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYP3A5 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CYP3A5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB).
WB~~1:1000. Researchers should empirically determine the suitability of the CYP3A5 cyp3a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CYP3A5 cyp3a5 product has the following accession number(s) (GI #4503231) (NCBI Accession #NP_000768.1) (Uniprot Accession #P20815). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CYP3A5 Antibody (Center) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of CYP3A5 Antibody (Center) in 293 cell line lysates (35ug/lane). CYP3A5 (arrow) was detected using the purified Pab.)
Western Blot (WB) CYP3A5.

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP3A5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC) CYP3A5.

Flow Cytometry (FC/FACS) (CYP3A5 Antibody (Center) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS) CYP3A5.

CYP3A5 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione.

Antigen Type: Synthetic Peptide. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CYP3A5 are readily searchable from our website. Different antibodies against the same target such as CYP3A5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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