anti-cyp8b1 antibody product blog
Tags: Antibody; Polyclonal Antibody; CYP8B1; anti-cyp8b1 antibody;
The cyp8b1 n/a (Catalog #MBS630437) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYP8B1, CT (Sterol 12-alpha-hydroxylase, 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, CYPVIIIB1, Cytochrome P450 8B1, Sterol 12-alpha-hydroxylase, 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase, CYP12) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CYP8B1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the cyp8b1 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The cyp8b1 n/a product has the following accession number(s) (GI #147899916) (NCBI Accession #NP_001079792.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CYP8B1, CT (Sterol 12-alpha-hydroxylase, 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, CYPVIIIB1, Cytochrome P450 8B1, Sterol 12-alpha-hydroxylase, 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase, CYP12) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of CYP8B1 Antibody (C-term) in K562 cell line lysates (35ug/lane). CYP8B1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP8B1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (CYP8B1 Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CYP8B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless.
Immunogen: Synthetic peptide selected from the C-terminal region of human CYP8B1 (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing cyp8b1 are readily searchable from our website. Different antibodies against the same target such as cyp8b1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.