anti-cypD antibody product blog
Tags: Antibody; Polyclonal Antibody; CYPD; anti-CYPD antibody;
The cypD n/a (Catalog #MBS628716) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYPD, NT (Cyclophilin-related Protein, Cyclophilin-40, CYP-40, 40kD Peptidyl-prolyl Cis-trans Isomerase, Rotamase D, Peptidyl-prolyl Cis-trans Isomerase D, PPIase D, CYP40, PPID) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CYPD can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the cypD n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The cypD n/a product has the following accession number(s) (GI #337747719). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CYPD, NT (Cyclophilin-related Protein, Cyclophilin-40, CYP-40, 40kD Peptidyl-prolyl Cis-trans Isomerase, Rotamase D, Peptidyl-prolyl Cis-trans Isomerase D, PPIase D, CYP40, PPID) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of PPID Antibody (N-term) in NIH-3T3 cell line lysates (35ug/lane). PPID (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with ETFB Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (PPID Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of PPID (arrow) using rabbit polyclonal PPID Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPID gene.)
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Immunogen: Synthetic peptide selected from the N-terminal region of human PPID (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing cypD are readily searchable from our website. Different antibodies against the same target such as cypD may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.