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anti-DCK antibody product blog

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Posted on 2017-08-22 23:33:09 by mybiosource_staff
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Tags: Antibody; DCK; anti-DCK antibody; Polyclonal Antibody;
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The DCK dck (Catalog #MBS127643) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DCK Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s DCK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the DCK dck for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The DCK dck product has the following accession number(s) (GI #118447) (NCBI Accession #P27707.1) (Uniprot Accession #P27707). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DCK Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using DCK Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.)
Western Blot (WB) DCK.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat kidney using DCK antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) DCK.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human vermiform appendix using DCK antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) DCK.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human gastric cancer using DCK antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) DCK.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human uterine cancer using DCK antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) DCK.

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells using DCK antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF) DCK.

Deoxycytidine kinase (dCK) is required for the phosphorylation of several deoxyribonucleoside analogues that are widely employed as chemotherapeutic agents(PMID:12628850). It is also a key enzyme in the phosphorylation of a variety of antineoplastic and antiviral nucleoside analogs including cytosine arabinoside (araC) and dideoxycytidine (ddCyd); deficiency of deoxycytidine kinase activity mediates resistance to these drugs(PMID:1996353). This protein can exsit as a homodimer(PMID:16421443). This antibody is specific to DCK.

Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human DCK
Calculated Molecular Weight: 31kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DCK are readily searchable from our website. Different antibodies against the same target such as DCK may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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