anti-DCLK3 antibody product blog
Tags: Antibody; Polyclonal Antibody; DCLK3; anti-DCLK3 antibody;
The DCLK3 dclk3 (Catalog #MBS151422) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DCLK3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s DCLK3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF).DCLK3 antibody can be used for detection of DCLK3 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunoflourescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the DCLK3 dclk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DCLK3 dclk3 product has the following accession number(s) (GI #149589021) (NCBI Accession #NP_208382) (Uniprot Accession #Q9C098). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DCLK3 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of DCLK3 in K562 cell lysate with DCLK3 antibody at (A) 1 and (B) 2 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of DLCK3 in human brain tissue with DLCK3 antibody at 20 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of DCLK3 in mouse brain tissue with DCLK3 Antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of DCLK3 in mouse brain tissue with DCLK3 Antibody at 20 μg/mL.
Green: DCLK3 antibody (5637)
Red: Phylloidin staining
Blue: DAPI staining)
DCLK3 Antibody: DCLK3 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK3 mRNA, like that of the homologous DCLK1 and DCLK3, is highly expressed in adult brain, but only DCLK3 transcripts are present in liver and kidney, suggesting that DCLK3 may play other roles than in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization.
Conjugate: Unconjugated
Immunogen: DCLK3 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human DCLK3.
Buffer: DCLK3 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DCLK3 are readily searchable from our website. Different antibodies against the same target such as DCLK3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.