anti-DCT antibody product blog
Tags: Antibody; Polyclonal Antibody; DCT; anti-DCT antibody;
The DCT dct (Catalog #MBS9206412) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DCT Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DCT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50. Researchers should empirically determine the suitability of the DCT dct for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DCT dct product has the following accession number(s) (GI #193788640) (NCBI Accession #NP_001123361.1) (Uniprot Accession #P40126). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DCT Antibody (N-term) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of DCT Antibody (N-term) in K562(lane 1), A375(lane 2), Ramos(lane 3) cell line lysates (35ug/lane). DCT (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DCT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (DCT Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
DCT is involved in regulating eumelanin and phaeomelanin levels.
Function : Catalyzes the conversion of L-dopachrome into 5,6- dihydroxyindole-2-carboxylic acid (DHICA) (PubMed:8306979). Involved in regulating eumelanin and phaeomelanin levels.
Cellular Location : Melanosome membrane; Single-pass type I membrane protein. Melanosome {ECO:0000250|UniProtKB:P29812}. Note=Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38 {ECO:0000250|UniProtKB:P29812}. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DCT are readily searchable from our website. Different antibodies against the same target such as DCT may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Bone, Brain, Connective Tissue, Embryonic Tissue, Eye, Heart, Intestine, Kidney, Lung, Skin tissues are correlated with this protein. DCT also interacts with the following gene(s): DDT, GAPDH, MITF, OTX2, PMEL, SOX9, TYRP1.