anti-DDX3X antibody product blog
Tags: Antibody; DDX3X; anti-DDX3X antibody; Polyclonal Antibody; DDX3;
The DDX3X ddx3x (Catalog #MBS150245) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDX3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s DDX3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).DDX3 antibody can be used for the detection of DDX3 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the DDX3X ddx3x for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DDX3X ddx3x product has the following accession number(s) (GI #3523150) (NCBI Accession #AAC34298) (Uniprot Accession #O00571). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DDX3 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 0.5 and (B) 1 μg/mL.)
Immunocytochemistry (IHC) (Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of DDX3 in HepG2 cells with DDX3 antibody at 20 μg/mL.)
DDX3 Antibody: DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.
Conjugate: Unconjugated
Immunogen: DDX3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human DDX3.
Buffer: DDX3 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DDX3X are readily searchable from our website. Different antibodies against the same target such as DDX3X may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.