anti-DFFA antibody product blog
Tags: Antibody; DFFA; anti-DFFA antibody; Polyclonal Antibody;
The DFFA dffa (Catalog #MBS9211495) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DFFA Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DFFA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).WB~~1:1000. Researchers should empirically determine the suitability of the DFFA dffa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DFFA dffa product has the following accession number(s) (GI #4758148) (NCBI Accession #NP_004392.1) (Uniprot Accession #O00273). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DFFA Antibody (C-term) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of DFFA (arrow) using rabbit polyclonal DFFA Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DFFA gene.)
Western Blot (WB) (Western blot analysis of DFFA Antibody (C-term) in K562 cell line lysates (35ug/lane). DFFA (arrow) was detected using the purified Pab.)
DFFA is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
Antigen Type: Synthetic Peptide. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DFFA are readily searchable from our website. Different antibodies against the same target such as DFFA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Bone Marrow, Connective Tissue, Eye, Heart, Kidney, Liver, Muscle, Vascular tissues are correlated with this protein. DFFA also interacts with the following gene(s): CASP3, CIDEB, CIDEC, DFFB, MYC.