anti-Dffb antibody product blog
Tags: Antibody; DFFB; anti-DFFB antibody; Polyclonal Antibody; DFF40;
The Dffb dffb (Catalog #MBS150231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DFF40 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DFF40 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).DFF40 antibody can be used for detection of DFF40 by Western blot at 0.5 mug/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Positive Control:
MBS151739 K562 Cell Lysate
MBS151729 Jurkat Cell Lysate. Researchers should empirically determine the suitability of the Dffb dffb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Dffb dffb product has the following accession number(s) (GI #160948620) (NCBI Accession #NP_031885) (Uniprot Accession #O54788). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DFF40 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of DFF40 in Jurkat (J) and K562 (K) whole cell lysate with DFF40 antibody at 1:500 dilution.)
Immunocytochemistry (IHC) (Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of DFF40 in Jurkat cells with DFF40 antibody at 20 μg/mL.)
DFF40 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD for caspase activated deoxyribonuclease. The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
Conjugate: Unconjugated
Immunogen: DFF40 antibody was raised against a 16 amino acid peptide near the amino terminus of human DFF40.
Buffer: DFF40 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Dffb are readily searchable from our website. Different antibodies against the same target such as Dffb may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.