anti-Dffb antibody product blog
Tags: Antibody; DFFB; anti-DFFB antibody; Polyclonal Antibody; CAD;
The Dffb dffb (Catalog #MBS150588) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CAD Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CAD can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).CAD antibody antibody can be used for detection of CAD by Western blot at 0.5 mug/mL. A 40 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 mug/mL. For immunofluorescence start at 10 mug/mL. Researchers should empirically determine the suitability of the Dffb dffb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Dffb dffb product has the following accession number(s) (GI #20137695) (NCBI Accession #O54788.1) (Uniprot Accession #O54788). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CAD Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of CAD in mouse lung (L) and kidney (K) tissue lysates with CAD antibody at 1:500 dilution.)
Immunohistochemistry (IHC) (Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 1 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of CAD in Mouse Kidney cells with CAD antibody at 10 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 5 μg/ml.)
Immunofluorescence (IF) (Immunofluorescence of CAD in mouse kidney tissue with CAD antibody at 5 μg/ml.
Green: CAD antibody (2011)
Red: Phylloidin staining
Blue: DAPI staining)
CAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
Conjugate: Unconjugated
Immunogen: CAD antibody was raised against a 16 amino acid peptide near the carboxy terminus of murine CAD.
Buffer: CAD Antibody is supplied in PBS containing 0.02% sodium azide.