anti-DGKQ antibody product blog
Tags: Antibody; Polyclonal Antibody; DGKQ; anti-DGKQ antibody;
The DGKQ dgkq (Catalog #MBS627875) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DGKQ, CT (Diacylglycerol Kinase theta, DAG Kinase theta, Diglyceride Kinase theta, DGK-theta, DAGK4) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DGKQ can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC).Suitable for use in ELISA and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the DGKQ dgkq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DGKQ dgkq product has the following accession number(s) (GI #40806175) (NCBI Accession #NP_001338.2) (Uniprot Accession #P52824). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
DGKQ, CT (Diacylglycerol Kinase theta, DAG Kinase theta, Diglyceride Kinase theta, DGK-theta, DAGK4)
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DGKQ, CT (Diacylglycerol Kinase theta, DAG Kinase theta, Diglyceride Kinase theta, DGK-theta, DAGK4) with the following immunoassay(s):
Western Blot (WB) (DGKQ Antibody (R927) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DGKQ antibody detected the DGKQ protein (arrow).)
Immunohistochemistry (IHC) (DGKQ Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DGKQ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (DGKQ Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Diacylglycerol (DAG) is an allosteric activator of protein kinase C. DAG also participates in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1. DAG is also involved in the synthesis of phospholipids and triacylglycerols. Tight regulation of DAG levels is achieved via DAG kinases (DGKs), which remove DAG by phosphorylate it to phosphatidic acid.The predicted 882aa human DGKQ (DGK-theta) protein is 90% identical in sequence to the rat homolog. DGK-theta is comprised of a pleckstrin homology domain and the conserved DGK putative catalytic domain. In contrast to other DGK isotypes, DGK-theta contains 3 rather than 2 cysteine-rich zinc-binding domains, an N-terminal proline- and glycine-rich region, and a RAS-associating domain. Highest tissue expression in the rat is in the brain. Defects in eye-specific DAGK genes cause retinal degeneration in Drosophila; thus, DAGK genes are candidates for human eye disease.
Immunogen: Synthetic peptide selected from the C-terminal region of human DGKQ (KLH).