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anti-DKK4 antibody product blog

Posted on 2015-08-16 15:42:18 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; DKK4; anti-DKK4 antibody;
The DKK4 dkk4 (Catalog #MBS631285) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DKK4, CT (Dickkopf-related Protein 4, Dickkopf-4, Dkk-4) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DKK4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the DKK4 dkk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The DKK4 dkk4 product has the following accession number(s) (GI #7657025) (NCBI Accession #NP_055235.1) (Uniprot Accession #Q9UBT3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

DKK4, like DKK1, DKK2, and DKK3, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.

DKK4 dkk4

Immunogen: Synthetic peptide selected from the C-terminal region of human DKK4 (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DKK4 are readily searchable from our website. Different antibodies against the same target such as DKK4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain, Embryonic Tissue, Intestine, Liver, Mammary Gland, Stomach tissues are correlated with this protein. Adenocarcinoma, Carcinoma, Carcinoma, Renal Cell, Colonic Neoplasms, Colorectal Neoplasms, Female Urogenital Diseases, Kidney Neoplasms, Mammary Neoplasms, Experimental, Neoplasms, Stomach Neoplasms are some of the diseases may be linked to DKK4, CT (Dickkopf-related Protein 4, Dickkopf-4, Dkk-4).
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