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anti-DNA antibody product blog

Posted on 2016-04-09 15:27:59 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; DNA; anti-DNA antibody; DNA polymerase gamma;
The DNA polg (Catalog #MBS837728) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNA polymerase gamma antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DNA polymerase gamma can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB).
IHC-P: 1:100-1:500
WB: 1:1000-1:10000. Researchers should empirically determine the suitability of the DNA polg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The DNA polg product has the following accession number(s) (GI #1399956) (NCBI Accession #AAC50712.1) (Uniprot Accession #P54098). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DNA polymerase gamma antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of A. 50 ug Rat testis lysate/extract using a 5 % SDS PAGE gel and DNA polymerase gamma antibody at a dilution of 1:1000)
Western Blot (WB) DNA.

Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: MCF-7) using a 5 % SDS PAGE gel and DNA polymerase gamma antibody at a dilution of 1:1000)
Western Blot (WB) DNA.

Immunofluorescence (IF) (Immunofluorescence analysis of methanol-fixed HeLa, using DNA polymerase gamma antibody at 1:200 dilution.)
Immunofluorescence (IF) DNA.

Immunohistochemistry (IHC) (Immunohistochemical staining of paraffin-embedded Skeletal Muscle using DNA polymerase gamma antibody)
Immunohistochemistry (IHC) DNA.

Affinity purified Rabbit polyclonal DNA polymerase gamma antibody.

Biological Significance: Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Immunogen: DNA polymerase gamma antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 779 and 1231 of DNA polymerase gamma. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DNA are readily searchable from our website. Different antibodies against the same target such as DNA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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