|The DNA ligase 1 lig1 (Catalog #MBS7005245) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human DNA ligase 1 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DNA ligase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC).
Recommended dilution: IHC: 1:20-1:200. Researchers should empirically determine the suitability of the DNA ligase 1 lig1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DNA ligase 1 lig1 product has the following accession number(s) (GI #4557719) (NCBI Accession #NP_000225.1) (Uniprot Accession #P18858). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti-human DNA ligase 1 polyclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7005245 at dilution 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of A549 cells using MBS7005245 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.
Immunogen: Recombinant human DNA ligase 1 protein. Conjugation: Non-conjugated
Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-20222 / sc-20224 / sc-366180. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DNA ligase 1 are readily searchable from our website. Different antibodies against the same target such as DNA ligase 1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Brain, Intestine, Kidney, Liver, Lung, Muscle, Prostate, Skin, Stomach tissues are correlated with this protein. Anemia, Breast Neoplasms, Carcinoma, Squamous Cell, Cognition Disorders, Heart Diseases, Infertility, Female, Lung Diseases, Lung Neoplasms, Poisoning, Uterine Cervical Neoplasms are some of the diseases may be linked to Rabbit anti-human DNA ligase 1 polyclonal Antibody. DNA ligase 1 also interacts with the following gene(s): APEX1, FEN1, PCNA, POLD1.