anti-DNAJB1 antibody product blog
Tags: Antibody; HSP40; Polyclonal Antibody; anti-DNAJB1 antibody; DNAJB1;
The DNAJB1 dnajb1 (Catalog #MBS806311) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hsp40 Antibody: ATTO 594 reacts with Human, Mouse, Rat, African Green Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s Hsp40 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP).1:1500-2000 (WB). Researchers should empirically determine the suitability of the DNAJB1 dnajb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DNAJB1 dnajb1 product has the following accession number(s) (GI #5453690) (NCBI Accession #NP_006136.1) (Uniprot Accession #P25685). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Hsp40 Antibody: ATTO 594 with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HSP40 protein using Rabbit Anti-HSP40 Polyclonal Antibody (SPC-100). Primary Antibody: Rabbit Anti-HSP40 Polyclonal Antibody (SPC-100) at 1:1000.)
Background Info: Detects an ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Also detects recombinant Hsp40.
Scientific Background: DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s. Hsp40, also known as HDJ1 (6), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-5). Hsp 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK (2).
Conjugate: ATTO 594. Storage Buffer: Rabbit antiserum. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DNAJB1 are readily searchable from our website. Different antibodies against the same target such as DNAJB1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.