anti-DNMT3B antibody product blog
Tags: Antibody; DNMT3B; anti-DNMT3B antibody; Polyclonal Antibody;
The DNMT3B dnmt3b (Catalog #MBS126366) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNMT3B Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s DNMT3B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).WB: 1:200 - 1:1000. Researchers should empirically determine the suitability of the DNMT3B dnmt3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DNMT3B dnmt3b product has the following accession number(s) (GI #17375667) (NCBI Accession #Q9UBC3.1) (Uniprot Accession #Q9UBC3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DNMT3B Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using DNMT3B antibody at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011].
Species: Human
Route: Synthetic Peptide. Immunogen: A synthetic peptide of human DNMT3B
Calculated Molecular Weight: 96kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DNMT3B are readily searchable from our website. Different antibodies against the same target such as DNMT3B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Lymph Node, Prostate, Uncharacterized Tissue, Uterus tissues are correlated with this protein. The following patways have been known to be associated with this gene. DNMT3B also interacts with the following gene(s): CBX5, DNMT1, DNMT3A, DNMT3L, EZH2, HDAC1, NCAPG, SIN3A, SMARCA5, UHRF1.