|The E2F1 e2f1 (Catalog #MBS126088) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The E2F1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s E2F1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100. Researchers should empirically determine the suitability of the E2F1 e2f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The E2F1 e2f1 product has the following accession number(s) (GI #400928) (NCBI Accession #Q01094.1) (Uniprot Accession #Q01094). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the E2F1 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of HeLa cells, using E2F1 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
DNA tumor viruses appear to have transforming activity due to their binding and inactivating to the RB1 gene products, and transcription facotr E2F, which is first identified as a DNA-binding protein essential for E1A-dependent activation of the advenovirus E2 promoter. The complex DRTF1/E2F involves in the control of cell-cycle progression from G1 to S phase. E2F1, one of E2F preferentially binds to RB1 protein in a cell-cycle dependent manner. It mediates both cell proliferation and p53-dependent apoptosis.
Route: Synthetic Peptide. Immunogen: Recombinant protein of human E2F1
Calculated Molecular Weight: 47kD. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing E2F1 are readily searchable from our website. Different antibodies against the same target such as E2F1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Embryonic Tissue, Kidney, Liver, Lung, Muscle, Prostate, Skin tissues are correlated with this protein. Adenocarcinoma, Breast Neoplasms, Carcinoma, Hepatocellular, Disease Models, Animal, Liver Diseases, Liver Neoplasms, Lung Neoplasms, Neoplasms, Experimental, Nervous System Diseases, Prostatic Neoplasms are some of the diseases may be linked to E2F1 Polyclonal Antibody.