|The E2f1 e2f1 (Catalog #MBS627269) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The E2F1, phosphorylated (Ser337) (Transcription Factor E2F1, E2F-1, Retinoblastoma Binding Protein 3, RBBP-3, PRB-binding Protein E2F-1, PBR3, Retinoblastoma-associated Protein 1, RBAP-1, RBBP3) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s E2F1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the E2f1 e2f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The E2f1 e2f1 product has the following accession number(s) (GI #405524) (NCBI Accession #AAA83217.1) (Uniprot Accession #Q61501). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser337 of human E2F1 (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing E2f1 are readily searchable from our website. Different antibodies against the same target such as E2f1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.