anti-EBF1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-EBF1 antibody; EBF1; early B-cell factor 1;
The EBF1 ebf1 (Catalog #MBS2027769) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Early B-Cell Factor 1 (EBF1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Early B-Cell Factor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA), IP, IF, FCM.Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the EBF1 ebf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The EBF1 ebf1 product has the following accession number(s) (GI #31415878) (NCBI Accession #NP_076870.1) (Uniprot Accession #Q9UH73). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- DP VIIDRFFLKF FLKCNQNCLK NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH GRRARRLDPS EGTPSYLEHA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVIFGTMLV WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGTPG RFIYTALNEP TIDYGFQRLQ KVIPRHPGDP ERLPKEVILK RAADLVEALY GMPHNNQEII LKRAADIAEA LYSVPRNHNQ LPALANTSVH.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Antibody to Early B-Cell Factor 1 (EBF1) with the following immunoassay(s):
Western Blot (WB) (Western Blot: Sample: Mouse Sp2/0 cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human EBF1 Antibody Second Ab: 0.2ug/mL�HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Western Blot (WB) (Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Heart tissue))
Knockout Validation (Knockout Validation: Lane 1: Wild-type Raji cell lysate; Lane 2: EBF1 knockout Raji cell lysate; Predicted MW: 64kDa Observed MW: 60kDa Primary Ab: 3ug/ml Rabbit Anti-Human EBF1 Antibody Second Ab: 0.2ug/mL�HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Fragment: EBF1 (Asp179~Ser451)
Organism Species: Homo sapiens (Human)
Source: Polyclonal antibody preparation
Cross Reactivity: Human, Rat. Conjugate: No Conjugate
Immunogen: Recombinant Early B-Cell Factor 1 (EBF1)
Traits: Liquid
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2055011. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing EBF1 are readily searchable from our website. Different antibodies against the same target such as EBF1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.