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anti-EDA antibody product blog

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Posted on 2015-09-21 14:51:26 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; EDA; anti-EDA antibody;
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The EDA eda (Catalog #MBS127421) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EDA Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s EDA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the EDA eda for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The EDA eda product has the following accession number(s) (GI #6166135) (NCBI Accession #Q92838.2) (Uniprot Accession #Q92838). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the EDA Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using EDA antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.)
Western Blot (WB) EDA.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human uterine cancer using EDA Antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC) EDA.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat liver using EDA Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) EDA.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat pancreas using EDA Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) EDA.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse liver using EDA Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) EDA.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse skeletal muscle using EDA Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) EDA.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse heart using EDA Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) EDA.

The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene.

Species: Human
Route: Synthetic Peptide. Immunogen: A synthetic peptide of human EDA
Calculated Molecular Weight: 49kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing EDA are readily searchable from our website. Different antibodies against the same target such as EDA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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