anti-EIF2S3 antibody product blog
Tags: Antibody; Polyclonal Antibody; EIF2S3; anti-EIF2S3 antibody;
The EIF2S3 eif2s3 (Catalog #MBS7044557) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF2S3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s EIF2S3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the EIF2S3 eif2s3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The EIF2S3 eif2s3 product has the following accession number(s) (GI #4503507) (NCBI Accession #NP_001406.1) (Uniprot Accession #P41091). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the EIF2S3 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot
All lanes: �EIF2S3 antibody at 1.13ug/ml
Lane 1�: Hela whole cell lysate
Lane 2�: Colo320 whole cell lysate
Lane 3�: HL60 whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS7044557 at dilution 1:100)
As a subunit of eukaryotic initiation factor 2 (eIF2), involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity). Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage (By similarity).
Species: Human
Immunogen: Recombinant human Eukaryotic translation initiation factor 2 subunit 3 protein (C-300AA). Conjugate: Non-conjugated
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-131149 / sc-131150. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing EIF2S3 are readily searchable from our website. Different antibodies against the same target such as EIF2S3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.