anti-EPCAM antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-Epcam antibody; Epcam; TACSTD1;
The EPCAM epcam (Catalog #MBS127524) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TACSTD1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s TACSTD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the EPCAM epcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The EPCAM epcam product has the following accession number(s) (GI #160266056) (NCBI Accession #P16422.2) (Uniprot Accession #P16422). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TACSTD1 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using EPCAM antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung cancer using EPCAM antibody at dilution of 1:100 (40x lens).)
Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3).
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human TACSTD1
Calculated Molecular Weight: 35kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing EPCAM are readily searchable from our website. Different antibodies against the same target such as EPCAM may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Adenocarcinoma, Breast Neoplasms, Carcinoma, Carcinoma, Hepatocellular, Carcinoma, Squamous Cell, Liver Diseases, Liver Neoplasms, Neoplasm Metastasis, Neoplasms, Experimental, Ovarian Neoplasms are some of the diseases may be linked to TACSTD1 Polyclonal Antibody. Ascites, Blood, Intestine, Kidney, Liver, Lung, Lymph Node, Prostate, Stomach, Vascular tissues are correlated with this protein.