anti-ERAP1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-ERAP1 antibody; ERAP1;
The ERAP1 erap1 (Catalog #MBS153542) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERAP1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s ERAP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF).ERAP1 antibody can be used for detection of ERAP1 by Western blot at 1 - 2 mug/ml.
Optimal dilutions for each application to be determined by the researcher. Researchers should empirically determine the suitability of the ERAP1 erap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ERAP1 erap1 product has the following accession number(s) (GI #94818901) (NCBI Accession #NP_057526) (Uniprot Accession #Q9NZ08). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ERAP1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of ERAP1 in SK-N-SH cell lysate with ERAP1 antibody at (A) 1 and (B) 2 μg/ml.)
Immunohistochemistry (IHC) (Immunohistochemistry of ERAP1 in mouse brain tissue with ERAP1 antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of ERAP1 in mouse brain tissue with ERAP1 antibody at 20 μg/mL.)
The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1), a member of the peptidase M1 family, plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides (1,2). It is also designated as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1) (3). ERAP1 is localized to the lumen of the ER and induced by interferon. It may be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (3,4).
Conjugate: Unconjugated
Immunogen: ERAP1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ERAP.
Buffer: ERAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Homology: Predicted species reactivity based on immunogen sequence: Rat: (78%)
Positive Control: 1)MBS151737 SK-N-SH Cell lysate
. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ERAP1 are readily searchable from our website. Different antibodies against the same target such as ERAP1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.