anti-F11R antibody product blog
Tags: Antibody; Polyclonal Antibody; F11R; anti-F11R antibody;
The F11R f11r (Catalog #MBS126747) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The F11R Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s F11R can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the F11R f11r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The F11R f11r product has the following accession number(s) (GI #10720061) (NCBI Accession #Q9Y624.1) (Uniprot Accession #Q9Y624). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the F11R Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using F11R antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells using F11R antibody. Blue: DAPI for nuclear staining.)
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5\' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008].
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human F11R
Calculated Molecular Weight: 33kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing F11R are readily searchable from our website. Different antibodies against the same target such as F11R may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.