|The FBXO32 fbxo32 (Catalog #MBS126900) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FBXO32 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s FBXO32 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the FBXO32 fbxo32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The FBXO32 fbxo32 product has the following accession number(s) (GI #121943365) (NCBI Accession #Q498Y9) (Uniprot Accession #Q498Y9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the FBXO32 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using FBXO32 antibody at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat liver using FBXO32 antibody at dilution of 1:100 (40x lens).)
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human FBXO32
Calculated Molecular Weight: 41kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FBXO32 are readily searchable from our website. Different antibodies against the same target such as FBXO32 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.