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anti-FLT3LG antibody product blog

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Posted on 2017-12-21 15:33:19 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; FLT3LG; Flt3 LIGAND; anti-FLT3LG antibody;
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The FLT3LG flt3lg (Catalog #MBS176351) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Flt3 ligand antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Flt3 ligand can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the FLT3LG flt3lg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The FLT3LG flt3lg product has the following accession number(s) (GI #1706818) (NCBI Accession #P49771.1) (Uniprot Accession #P49771). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Flt3 ligand antibody with the following immunoassay(s):
Western Blot (WB) (Anti-Flt-3ligand antibody, MBS176351, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human Flt-3ligand, 40.7KD(162aa tag+P36-H235)
Lane 1: Recombinant Human Flt-3ligand Protein 10ng
Lane 2: Recombinant Human Flt-3ligand Protein 5ng )
Western Blot (WB) FLT3LG.

Description: Rabbit IgG polyclonal antibody for Fms-related tyrosine kinase 3 ligand(FLT3LG) detection. Tested with WB in Human.
Background: FLT3LG(FMS-Related Tyrosine Kinase 3 Ligand) also called FLT3 LIGAND, FL or FLT3L, is a protein which in humans is encoded by the FLT3LG gene. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt3 ligand(FL) is a hematopoietic four helical bundlecytokine. It is structurally homologous to stem cell factor(SCF) and colony stimulating facor 1(CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. Lyman et al.(1993) found that Flt3 ligand stimulated proliferation of hematopoietic progenitor cells isolated from mouse fetal liver or adult mouse bone marrow. Hannum et al.(1994) concluded that FL enhances the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells. Assays of C-terminally truncated FL proteins confirmed that the N-terminal half conferred FL activity. Depletion of the Pi3k -Mtor negative regulator Pten facilitated Flt3l-driven DC development in culture.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Flt3 ligand (206-220aa HWQRTRRRTPRPGEQ). Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FLT3LG are readily searchable from our website. Different antibodies against the same target such as FLT3LG may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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