|The FSTL3 fstl3 (Catalog #MBS176044) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FSTL3 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s FSTL3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the FSTL3 fstl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The FSTL3 fstl3 product has the following accession number(s) (GI #23821565) (NCBI Accession #O95633.1) (Uniprot Accession #O95633). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-FSTL3 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-FSTL3 antibody, MBS176044, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human FSTL3, 39.2KD(162aa tag+ L52-P251)
Lane 1: Recombinant Human FSTL3 Protein 10ng
Lane 2: Recombinant Human FSTL3 Protein 5ng )
Description: Rabbit IgG polyclonal antibody for Follistatin-related protein 3(FSTL3) detection. Tested with WB in Human, Mouse, Rat.
Background: FSTL3(Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins though to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. By Southern blot analysis of somatic cell hybrids and FISH, Hayette et al.(1998) localized the FSTL3 gene to chromosome 19p13. Using recombinant mouse Fstl3, Tsuchida et al.(2000) found that Fstl3bound both activin and BMP2 and had a higher affinity for activin. Overexpression of Fstl3 inhibited BMP2-induced cell signaling in a reporter assay. In addition to the translocation in a case of B-cell chronic lymphocytic leukemia from which FSTL3 was isolated, Hayette et al.(1998) also observed rearrangement of the FSTL3 gene in a case of B-cell non-Hodgkin lymphoma and in a case of B-cell mantle zone lymphoma, suggesting that FSTL3 may be involved in the leukemogenesis process.
Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human FSTL3 (145-159aa ECELRAARCRGHPDL), different from the related rat and mouse sequences by one amino acid. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FSTL3 are readily searchable from our website. Different antibodies against the same target such as FSTL3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.