anti-FURIN antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-FURIN antibody; FURIN;
The FURIN furin (Catalog #MBS9127539) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s FURIN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the FURIN furin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The FURIN furin product has the following accession number(s) (GI #120611) (NCBI Accession #P09958.2) (Uniprot Accession #P09958). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the FURIN with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using FURIN antibody (MBS9127539) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver cancer using FURIN antibody (MBS9127539) at dilution of 1:100 (40xlens).)
Immunofluorescence (IF) (Immunofluorescence analysis of A549 cells using FURIN Rabbit pAb (MBS9127539) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF) (Immunofluorescence analysis of NIH-3T3 cells using FURIN Rabbit pAb (MBS9127539) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF) (Immunofluorescence analysis of U-2 OS cells using FURIN Rabbit pAb (MBS9127539) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. The product of this gene is one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pronerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140 and may play a role in tumor progression. This gene is located in close proximity to family member proprotein convertase subtilisin/kexin type 6 and upstream of the FES oncogene. Alternative splicing results in multiple transcript variants.